Cloning and characterization of defense-related genes from Populus szechuanica infected with rust fungus Melampsora larici-populina.
Identifieur interne : 001966 ( Main/Exploration ); précédent : 001965; suivant : 001967Cloning and characterization of defense-related genes from Populus szechuanica infected with rust fungus Melampsora larici-populina.
Auteurs : Z J Chen [République populaire de Chine] ; Z M Cao [République populaire de Chine] ; Z D Yu [République populaire de Chine] ; D. Yu [République populaire de Chine]Source :
- Genetics and molecular research : GMR [ 1676-5680 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Maladies des plantes, Phenylalanine ammonia-lyase, Populus.
- microbiologie : Populus.
- pathogénicité : Basidiomycota.
- Interactions hôte-pathogène.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Phenylalanine Ammonia-Lyase.
- genetics : Plant Diseases, Populus.
- microbiology : Populus.
- pathogenicity : Basidiomycota.
- Host-Pathogen Interactions.
Abstract
Characterization of defense-related genes is critical for breeding disease-resistant poplar varieties and for better management and control of leaf rust disease. In the present study, full-length cDNAs of five Populus szechuanica defense-related (PsDR) genes, pathogen-related protein 1 (PsPR1), β-1,3-glucanase (PsGns), thaumatin-like protein 1 (PsTLP1), thaumatin-like protein 2 (PsTLP2), and phenylalanine ammonia-lyase (PsPAL), were cloned from the leaves of P. szechuanica infected with Melampsora larici-populina (MLP). PsPR1 (728 bp), PsGns (1189 bp), PsTLP1 (929 bp), PsTLP2 (885 bp), and PsPAL (2586 bp) were predicted to encode 161, 347, 245, 225, and 711 amino acid residue-containing proteins with isoelectric points of 8.53, 4.96, 4.51, 7.32, and 5.87, respectively. Moreover, the deduced PsDR proteins displayed more than 90% similarity to proteins from other Populus species. In response to the avirulent isolate, Sb052, and the virulent isolate, Th053, of MLP, the expression of PsDR genes was rapidly up-regulated in the leaves of P. szechuanica, peaked at 2 or 7 days post-inoculation (dpi), with levels in the incompatible interaction being higher than those in the compatible interaction. Meanwhile, the expression of PsDR genes (except for PsGns) was also differentially up-regulated at 3, 7, or 18 dpi in the petioles of the infected leaves, leaves next to the inoculated leaves, and in the top buds of the infected plants, respectively, compared to that at 0 dpi. These results suggest that these PsDR genes could play distinctive roles in the defense response of poplar against rust infection.
DOI: 10.4238/gmr.15017314
PubMed: 26909999
Affiliations:
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Yu</name><affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northwest A&F University, Yangling, Shaanxi, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Northwest A&F University, Yangling, Shaanxi</wicri:regionArea><wicri:noRegion>Shaanxi</wicri:noRegion></affiliation></author></analytic><series><title level="j">Genetics and molecular research : GMR</title><idno type="eISSN">1676-5680</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (pathogenicity)</term><term>Host-Pathogen Interactions (MeSH)</term><term>Phenylalanine Ammonia-Lyase (genetics)</term><term>Plant Diseases (genetics)</term><term>Populus (genetics)</term><term>Populus (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Basidiomycota (pathogénicité)</term><term>Interactions hôte-pathogène (MeSH)</term><term>Maladies des plantes (génétique)</term><term>Phenylalanine ammonia-lyase (génétique)</term><term>Populus (génétique)</term><term>Populus (microbiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Phenylalanine Ammonia-Lyase</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Diseases</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Maladies des plantes</term><term>Phenylalanine ammonia-lyase</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Basidiomycota</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Host-Pathogen Interactions</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Interactions hôte-pathogène</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Characterization of defense-related genes is critical for breeding disease-resistant poplar varieties and for better management and control of leaf rust disease. In the present study, full-length cDNAs of five Populus szechuanica defense-related (PsDR) genes, pathogen-related protein 1 (PsPR1), β-1,3-glucanase (PsGns), thaumatin-like protein 1 (PsTLP1), thaumatin-like protein 2 (PsTLP2), and phenylalanine ammonia-lyase (PsPAL), were cloned from the leaves of P. szechuanica infected with Melampsora larici-populina (MLP). PsPR1 (728 bp), PsGns (1189 bp), PsTLP1 (929 bp), PsTLP2 (885 bp), and PsPAL (2586 bp) were predicted to encode 161, 347, 245, 225, and 711 amino acid residue-containing proteins with isoelectric points of 8.53, 4.96, 4.51, 7.32, and 5.87, respectively. Moreover, the deduced PsDR proteins displayed more than 90% similarity to proteins from other Populus species. In response to the avirulent isolate, Sb052, and the virulent isolate, Th053, of MLP, the expression of PsDR genes was rapidly up-regulated in the leaves of P. szechuanica, peaked at 2 or 7 days post-inoculation (dpi), with levels in the incompatible interaction being higher than those in the compatible interaction. Meanwhile, the expression of PsDR genes (except for PsGns) was also differentially up-regulated at 3, 7, or 18 dpi in the petioles of the infected leaves, leaves next to the inoculated leaves, and in the top buds of the infected plants, respectively, compared to that at 0 dpi. These results suggest that these PsDR genes could play distinctive roles in the defense response of poplar against rust infection. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26909999</PMID><DateCompleted><Year>2016</Year><Month>12</Month><Day>30</Day></DateCompleted><DateRevised><Year>2016</Year><Month>12</Month><Day>31</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1676-5680</ISSN><JournalIssue CitedMedium="Internet"><Volume>15</Volume><Issue>1</Issue><PubDate><Year>2016</Year><Month>Feb</Month><Day>22</Day></PubDate></JournalIssue><Title>Genetics and molecular research : GMR</Title><ISOAbbreviation>Genet Mol Res</ISOAbbreviation></Journal><ArticleTitle>Cloning and characterization of defense-related genes from Populus szechuanica infected with rust fungus Melampsora larici-populina.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.4238/gmr.15017314</ELocationID><Abstract><AbstractText>Characterization of defense-related genes is critical for breeding disease-resistant poplar varieties and for better management and control of leaf rust disease. In the present study, full-length cDNAs of five Populus szechuanica defense-related (PsDR) genes, pathogen-related protein 1 (PsPR1), β-1,3-glucanase (PsGns), thaumatin-like protein 1 (PsTLP1), thaumatin-like protein 2 (PsTLP2), and phenylalanine ammonia-lyase (PsPAL), were cloned from the leaves of P. szechuanica infected with Melampsora larici-populina (MLP). PsPR1 (728 bp), PsGns (1189 bp), PsTLP1 (929 bp), PsTLP2 (885 bp), and PsPAL (2586 bp) were predicted to encode 161, 347, 245, 225, and 711 amino acid residue-containing proteins with isoelectric points of 8.53, 4.96, 4.51, 7.32, and 5.87, respectively. Moreover, the deduced PsDR proteins displayed more than 90% similarity to proteins from other Populus species. In response to the avirulent isolate, Sb052, and the virulent isolate, Th053, of MLP, the expression of PsDR genes was rapidly up-regulated in the leaves of P. szechuanica, peaked at 2 or 7 days post-inoculation (dpi), with levels in the incompatible interaction being higher than those in the compatible interaction. Meanwhile, the expression of PsDR genes (except for PsGns) was also differentially up-regulated at 3, 7, or 18 dpi in the petioles of the infected leaves, leaves next to the inoculated leaves, and in the top buds of the infected plants, respectively, compared to that at 0 dpi. These results suggest that these PsDR genes could play distinctive roles in the defense response of poplar against rust infection. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Z J</ForeName><Initials>ZJ</Initials><AffiliationInfo><Affiliation>College of Forestry, Northwest A&F University, Yangling, Shaanxi, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Forestry and Landscape Architecture, Guangzhou, Guangdong, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Z M</ForeName><Initials>ZM</Initials><AffiliationInfo><Affiliation>College of Forestry, Northwest A&F University, Yangling, Shaanxi, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Z D</ForeName><Initials>ZD</Initials><AffiliationInfo><Affiliation>College of Forestry, Northwest A&F University, Yangling, Shaanxi, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>D</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>College of Forestry, Northwest A&F University, Yangling, Shaanxi, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>02</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>Brazil</Country><MedlineTA>Genet Mol Res</MedlineTA><NlmUniqueID>101169387</NlmUniqueID><ISSNLinking>1676-5680</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>EC 4.3.1.24</RegistryNumber><NameOfSubstance UI="D010650">Phenylalanine Ammonia-Lyase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D054884" MajorTopicYN="N">Host-Pathogen Interactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010650" MajorTopicYN="N">Phenylalanine Ammonia-Lyase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>2</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>2</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>12</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26909999</ArticleId><ArticleId IdType="pii">gmr7314</ArticleId><ArticleId IdType="doi">10.4238/gmr.15017314</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Chen, Z J" sort="Chen, Z J" uniqKey="Chen Z" first="Z J" last="Chen">Z J Chen</name></noRegion><name sortKey="Cao, Z M" sort="Cao, Z M" uniqKey="Cao Z" first="Z M" last="Cao">Z M Cao</name><name sortKey="Chen, Z J" sort="Chen, Z J" uniqKey="Chen Z" first="Z J" last="Chen">Z J Chen</name><name sortKey="Yu, D" sort="Yu, D" uniqKey="Yu D" first="D" last="Yu">D. Yu</name><name sortKey="Yu, Z D" sort="Yu, Z D" uniqKey="Yu Z" first="Z D" last="Yu">Z D Yu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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